Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于定量检测大鼠血清、血浆及组织匀浆样本中Ⅲ型胶原（Col Ⅲ）的酶联免疫试剂盒，采用双抗体夹心法原理，检测灵敏度高，可在0.156 ng/mL至10 ng/mL的线性范围内精准测定目标蛋白含量。Ⅲ型胶原是细胞外基质的重要成分，广泛分布于皮肤、血管及内脏器官，其异常表达与组织纤维化、创伤修复及器官硬化等病理过程密切相关，是研究纤维化疾病模型和再生医学的关键生物标志物。本试剂盒配套预包被特异性抗体，实验操作流程简便，适用于实验室对大鼠纤维化疾病（如肝纤维化、肺纤维化）的机制研究、药物干预效果评估或组织工程中胶原代谢的动态监测。样本预处理步骤经过优化，支持直接检测稀释后的体液或匀浆液，无需复杂前处理，可在4小时内完成检测流程，满足科研场景中高通量、快速检测的需求。试剂盒包含标准品、检测抗体及显色液等核心组分，提供完整实验解决方案。
别名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
缩写：

Col Ⅲ
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	98
	Range %	92-104
1:4	Average %	95
	Range %	92-98
1:8	Average %	86
	Range %	80-94

回收率：

The recovery of rat Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	90	85-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 个评价

样品类型：血清

样品信息：大鼠

稀释比：1:50

产品评价： RAT collagen type3, elisa试剂盒检测大鼠血清，复孔的数据较稳定。试剂盒溶液给量充足。此外，还发现该试剂盒可以检测其他啮齿动物的血清样本，但OD值偏小。

By 杜老师