Pig Collagen Type Ⅲ,Col Ⅲ ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪Ⅲ型胶原（Col Ⅲ）酶联免疫试剂盒（货号：CSB-E06776p），用于定量检测血清、血浆及组织匀浆样本中的Ⅲ型胶原含量。Ⅲ型胶原作为细胞外基质的关键成分，广泛分布于皮肤、血管及内脏器官，与Ⅰ型胶原协同维持组织结构和弹性，其表达水平变化与纤维化疾病、创伤修复等生理病理过程密切相关。本试剂盒基于双抗体夹心法原理设计，检测范围为6.25-400 ng/mL，灵敏度高，可精准捕捉样本中Col Ⅲ的浓度差异。试剂盒操作便捷，适配常规实验室设备，适用于探究猪模型在纤维化机制、心血管疾病或皮肤再生等领域的科研需求，为组织工程、病理模型构建及分子机制研究提供可靠数据支持。试剂盒严格验证了血浆与组织匀浆样本的兼容性，确保实验结果的稳定性和重复性，是基础研究与转化医学中胶原代谢相关课题的理想工具。
别名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
缩写：

Col Ⅲ
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	91-103
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	93
	Range %	89-95

回收率：

The recovery of pig Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	92-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.