Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人Ⅲ型胶原（Col Ⅲ）酶联免疫检测试剂盒（货号：CSB-E04799h），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的Col Ⅲ蛋白。Ⅲ型胶原是细胞外基质的重要组成部分，主要分布于皮肤、血管和内脏器官，参与组织修复与纤维化过程，其表达水平与纤维化疾病、创伤愈合及器官硬化等病理生理机制密切相关。本试剂盒检测范围覆盖12.5 ng/mL至800 ng/mL，检测下限为12.5 ng/mL，实验需预先对血清或血浆样本进行离心处理，组织样本需制备为匀浆后检测。适用于体外研究Col Ⅲ在皮肤老化、肝纤维化、心血管疾病或组织工程修复等领域的表达调控，可为探索纤维化疾病分子机制或评估抗纤维化药物效果提供数据支持。试剂盒包含预包被抗体、标准品、检测抗体及显色系统，操作流程优化，可在4.5小时内完成检测，具有高特异性和稳定性，满足科研实验中对Col Ⅲ蛋白的精确定量需求。
别名：

Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
缩写：

Col Ⅲ
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-96
1:2	Average %	102
	Range %	97-105
1:4	Average %	92
	Range %	85-98
1:8	Average %	97
	Range %	91-102

回收率：

The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	80-94
EDTA plasma (n=4)	97	92-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.