Rabbit Thyroid Stimulating Hormone,TSH ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测兔子血清、血浆及组织匀浆中促甲状腺素（TSH）的酶联免疫试剂盒，采用双抗体夹心法原理设计，可精准检测0.5-20 μIU/mL浓度范围的TSH，灵敏度达0.25 μIU/mL。TSH作为垂体前叶分泌的糖蛋白激素，对调控甲状腺功能具有核心作用，其水平变化常关联甲状腺功能亢进或减退等内分泌研究。试剂盒包含预包被特异性抗体的96孔板及全套检测组分，适用于科研场景中甲状腺相关疾病的动物模型研究、药物干预对激素分泌的影响分析或基础内分泌机制探索。样本兼容性广泛，实验全程约需4小时完成，配套标准品可建立高线性标准曲线（R²≥0.99），为实验动物TSH水平监测提供可靠工具。本产品严格验证了批间重复性，适用于需要高灵敏度检测的实验室，特别推荐用于小动物内分泌系统研究的纵向观察或跨组学关联分析。
缩写：

TSH
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.5 μIU/mL-20 μIU/mL
灵敏度：

0.25 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	92-104
1:2	Average %	84
	Range %	80-88
1:4	Average %	94
	Range %	90-98
1:8	Average %	92
	Range %	87-96

回收率：

The recovery of rabbit TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	96	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.