Fish thyroid stimulating hormone(TSH) ELISA Kit

Fish thyroid stimulating hormone(TSH) ELISA Kit

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中文名称：

鱼促甲状腺素(TSH)酶联免疫试剂盒
货号：

CSB-EQ027261FI
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

鱼促甲状腺素(TSH)酶联免疫试剂盒（CSB-EQ027261FI）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的TSH含量。TSH即促甲状腺激素，是腺垂体分泌的促进甲状腺生长和机能的激素。其作用机制主要是调控甲状腺细胞的生长和甲状腺血液供应，促进甲状腺上皮细胞增生和甲状腺血液供应。在临床研究中，TSH水平可反映甲状腺功能状态，是诊断甲状腺疾病的重要指标。试剂盒检测范围为2.5 μIU/mL-40 μIU/mL，适用于实验室开展鱼类生理研究、内分泌干扰物效应评估、水产养殖健康监测等科研场景。该检测体系兼容多种样本类型，为研究鱼类甲状腺功能调控机制、环境污染物对内分泌系统的影响提供可靠工具，特别适用于水生生物毒理学实验和基础生物学研究中的甲状腺相关指标分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

tshb ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
缩写：

TSH
Uniprot No.：

P37240
种属：

Fish
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.5 μIU/mL-40 μIU/mL
灵敏度：

2.5 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	96-106
1:2	Average %	89
	Range %	84-94
1:4	Average %	94
	Range %	90-98
1:8	Average %	96
	Range %	91-101

回收率：

The recovery of fish TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-93
EDTA plasma (n=4)	95	89-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
40	0.234	0.228	0.231
20	0.511	0.502	0.507
10	0.888	0.893	0.891
5	1.743	1.727	1.735
2.5	2.557	2.458	2.508

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Thyroid and growth hormone endocrine disruption and mechanisms of homosalate and octisalate using wild-type, thrαa-/-, and dre-miR-499-/- zebrafish embryo/larvae Y Ka, I Lee, K Ji,Ecotoxicology and Environmental Safety,2024
Effects on thyroid hormone disruption by co-exposure to BEMT and TiO2 nanoparticle in embryo-larval zebrafish (Danio rerio) 박유진,서울대학교 대학원,2024
Thyroid Hormone and Lipid Metabolism Disruption of Di (2 propylheptyl) Phthalate (DPHP) in Different Life Stages of Zebrafish 박소은,서울대학교 대학원,2024
Thyroid endocrine disruption induced by [C8mim] Br: an integrated in vivo, in vitro, and in silico study P Wei,Aquatic toxicology,2023
Long-term low-dose oxytetracycline potentially leads to neurobehavioural changes Y Qiu,Ecotoxicology and environmental safety,2021
Zebrafish Embryonic Exposure to BPAP and Its Relatively Weak Thyroid Hormone-Disrupting Effects S Lee,Toxics,2020
Titanium dioxide nanoparticles enhanced thyroid endocrine disruption of pentachlorophenol rather than neurobehavioral defects in zebrafish larvae Lei L,Chemosphere,2020
Phenotypic plasticity as an important mechanism of cave colonization and adaptation in Astyanax cavefish Helena Bilandzija,bioRxiv,2019
Waterborne exposure to BPS causes thyroid endocrine disruption in zebrafish larvae Zhang DH.et al,PLoS One,2017
EFFECTS OF ROSEHIP EXTRACTS ON COMMON CARP (CYPRINUS CARPIO L.) TSH AND THYROID GLAND ACTIVITY H眉seyin Polat.et al,Fresenius Environmental Bulletin,2016
Waterborne exposure to bisphenol F causes thyroid endocrine disruption in zebrafish larvae Huang GM. et al,Chemosphere,2016
Mild hypothyroidism improves glucose tolerance in experimental type 2 diabetes Ashwini S et al,Chem Biol Interact,2015

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功能：

Indispensable for the control of thyroid structure and metabolism. May play some role in the biological processes of the immature fishes.
亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
组织特异性：

Pituitary gland. Higher levels seen in immature fishes than the mature fishes.
数据库链接：

UniGene: Omy.8084

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.