Canine Thyroid Stimulating Hormone,TSH ELISA Kit

Canine Thyroid Stimulating Hormone,TSH ELISA Kit

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中文名称：

犬促甲状腺素(TSH)酶联免疫试剂盒
货号：

CSB-E12959c
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

犬促甲状腺素(TSH)酶联免疫试剂盒（货号：CSB-E12959c）是一款基于竞争法原理开发的定量检测工具，适用于犬血清、血浆及组织匀浆样本中TSH水平的精准分析。TSH由垂体前叶分泌，通过调控甲状腺激素合成与释放维持机体代谢平衡，其水平异常与甲状腺功能亢进或减退等内分泌疾病密切相关，是评估犬类甲状腺健康的重要指标。本试剂盒线性检测区间覆盖0.625-10 μIU/mL，采用高特异性抗体实现低浓度样本的稳定检测，操作流程标准化，可在3小时内完成96孔板规模实验。该产品兼容多种生物样本类型，尤其适用于科研场景中甲状腺功能机制研究、药物对内分泌系统影响的评估，或疾病模型动物的激素水平动态监测，为兽医科研、宠物营养学及内分泌相关基础研究提供可靠数据支持。试剂盒组分包含预包被板、标准品、检测抗体及显色底物，配套详细操作手册确保实验可重复性。
别名：

TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
缩写：

TSH
Uniprot No.：

P54828
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 μIU/mL-10 μIU/mL
灵敏度：

0.625 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	95
	Range %	92-100
1:4	Average %	82
	Range %	80-84
1:8	Average %	92
	Range %	85-99

回收率：

The recovery of canine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	93	90-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μIU/ml	OD1	OD2	Average
10	0.195	0.188	0.192
5	0.334	0.353	0.344
2.5	0.531	0.514	0.523
1.25	1.017	1.102	1.060
0.625	2.138	2.320	2.229

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

STUDIES ON THYROID PROFILE IN DOGS WITH SPECIAL REFERENCE TO ASSESSMENT OF CANINE TSH AP DIPAKRAO,Thesis&Dissertation,2024

功能：

Indispensable for the control of thyroid structure and metabolism.
亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
数据库链接：

KEGG: cfa:403973

STRING: 9615.ENSCAFP00000014272

UniGene: Cfa.3841

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.