Rabbit Interferon α,IFN-α ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

兔子α干扰素(IFN-α)酶联免疫试剂盒（CSB-E07329Rb）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNA1含量。IFNA1 即α1型干扰素，其具有抗病毒、免疫调节等多种作用。在研究机制方面，它可与细胞表面受体结合，激活下游信号通路，诱导多种抗病毒蛋白产生，发挥抑制病毒复制和调节机体免疫反应等功能，是抗病毒和免疫相关研究的重要靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于生命科学研究中病毒侵染模型构建、免疫调节机制解析以及疫苗/抗病毒药物研发等场景，特别适用于兔科动物模型中天然免疫反应的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IFN-alpha，interferon alpha
缩写：

IFN-α
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	92
	Range %	88-98
1:4	Average %	95
	Range %	90-100
1:8	Average %	89
	Range %	86-94

回收率：

The recovery of rabbit IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	94	88-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.