Pig Interferon α,IFN-α ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪α干扰素（IFN-α）酶联免疫试剂盒（货号：CSB-E07328p）是基于夹心法原理开发的定量检测工具，专为科研领域设计，适用于检测血清、血浆、细胞培养上清及组织匀浆样本中的IFN-α含量。IFN-α作为天然免疫应答的关键因子，在抗病毒防御和免疫调节中发挥重要作用，其动态监测对研究猪类病毒感染机制、免疫应答评估及疫苗研发具有重要意义。本试剂盒检测灵敏度高，线性范围为7.8-500 pg/mL，最低检测限可达3.9 pg/mL，可在4.5小时内完成实验流程，支持多样本类型的高通量分析。通过预包被特异性抗体的微孔板实现靶标捕获，结合HRP标记检测抗体形成夹心复合物，经显色反应实现精准定量。该产品适用于体外实验研究，如病毒感染模型中的细胞因子动态分析、免疫调节剂效果评估或疫苗免疫原性研究，为科研人员提供稳定可靠的IFN-α浓度数据支持。
缩写：

IFN-α
Uniprot No.：

Q6VAB8
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-99
1:2	Average %	98
	Range %	91-105
1:4	Average %	96
	Range %	92-105
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of pig IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	97	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.