Rat Interferon α,IFN-α ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为大鼠α干扰素（IFN-α）检测设计的定量酶联免疫试剂盒（货号：CSB-E08637r），基于双抗体夹心法原理开发，适用于血清、血浆、细胞培养上清及组织匀浆样本的科研分析。IFN-α作为一种重要的细胞因子，在抗病毒免疫应答和免疫调节中发挥关键作用。该试剂盒检测灵敏度为31.25 pg/mL，线性范围覆盖31.25-2000 pg/mL，可在3小时内完成检测流程，仅需50μL样本即可完成实验。试剂盒包含预包被96孔板、系列浓度标准品、生物素标记检测抗体及TMB显色液等核心组分，通过标准曲线实现目标蛋白的精确定量，适用于体外免疫学研究、病毒感染模型评估或药物干预实验中IFN-α表达水平的动态监测。试剂盒组分需按说明书分存于-20℃（标准品）或2-8℃（其他试剂），开封后请在6个月内使用完毕。
别名：

Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit
缩写：

IFN-α
Uniprot No.：

P05011
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-91
1:2	Average %	92
	Range %	85-97
1:4	Average %	99
	Range %	94-104
1:8	Average %	103
	Range %	99-108

回收率：

The recovery of rat IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	96	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
亚细胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
数据库链接：

KEGG: rno:298210

STRING: 10116.ENSRNOP00000051043

UniGene: Rn.196548