Pig calprotectin (CALP) ELISA kit

产品详情

产品描述：

CUSABIO猪钙卫蛋白（CALP）酶联免疫检测试剂盒（货号CSB-EQ013485PI），适用于定量分析猪血清、血浆及组织匀浆样本中的CALP含量。钙卫蛋白属于S100钙结合蛋白家族，在炎症反应和先天免疫调控中发挥重要作用，其表达水平与中性粒细胞活化程度密切相关。该试剂盒基于竞争法原理设计，通过特异性抗体与样本中的CALP或标记抗原结合，检测信号强度与目标物浓度呈负相关，灵敏度覆盖0.312-20 ng/mL的线性检测范围。实验操作全程约需3-4小时，支持多样本类型检测，适用于动物疾病模型研究（如肠道炎症、感染性疾病）、免疫机制探索或药物开发中炎症标志物的动态监测。科研人员可通过该试剂盒精确评估猪体内CALP水平，为炎症相关研究提供可靠数据支持，特别适用于兽医病理学、比较医学及畜牧养殖领域的科研分析。
缩写：

CALP
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	91-102
1:200	Average %	85
	Range %	81-89
1:400	Average %	103
	Range %	98-107
1:800	Average %	87
	Range %	83-92

回收率：

The recovery of pig CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	88-97
EDTA plasma (n=4)	106	102-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.