Mouse calprotectin (CALP) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠钙卫蛋白(CALP)酶联免疫试剂盒（CSB-EQ013485MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CALP含量。材料未给出CALP靶点相关背景及研究机制的具体内容，无法为你总结。若有更多关于CALP靶点的详细信息，比如在哪些疾病研究中涉及、作用于机体的大致原理等，请补充后，我再进行总结。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于科研领域中对炎症相关动物模型（如结肠炎、败血症或关节炎模型）的病理机制研究，通过定量分析CALP水平，可评估中性粒细胞活化状态及组织炎症程度，为探索疾病发展、药物治疗效果或生物标志物筛选提供实验依据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

CALP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-88
1:8	Average %	89
	Range %	83-96

回收率：

The recovery of mouse CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	96	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.