Canine calprotectin (CALP) ELISA kit

中文名称：

犬钙卫蛋白(CALP)酶联免疫试剂盒
货号：

CSB-EQ013485DO
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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犬钙卫蛋白（CALP）酶联免疫试剂盒（货号：CSB-EQ013485DO）采用夹心法原理，可定量检测犬血清、血浆、组织匀浆及细胞裂解液等样本中的CALP蛋白水平，其检测灵敏度高，线性范围为6.25-400 ng/mL，为科研人员提供精准的定量分析工具。CALP是一种由中性粒细胞分泌的钙结合蛋白，广泛参与炎症反应调控，其表达水平与肠道炎症、感染性疾病等病理过程密切相关，是研究犬类炎症机制的重要生物标志物。该试剂盒采用预包被高特异性抗体，操作简便，兼容多样本类型，可满足实验室对犬类炎症相关疾病的机制研究、药物干预效果评估或动物模型分析等科研需求。通过标准化的检测流程，研究者可高效获取重复性良好的数据，适用于体外实验中探索CALP在犬免疫应答、组织损伤修复等领域的生物学功能。
缩写：

CALP
种属：

Canine
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

6.25 ng/mL-400 ng/mL
灵敏度：

1.56 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine CALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	102
	Range %	97-107
1:4	Average %	96
	Range %	85-99
1:8	Average %	103
	Range %	96-107

回收率：

The recovery of canine CALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	102	97-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	1.816	1.921	1.869	1.723
200	1.448	1.512	1.480	1.334
100	1.030	1.078	1.054	0.908
50	0.758	0.733	0.746	0.600
25	0.476	0.466	0.471	0.325
12.5	0.348	0.332	0.340	0.194
6.25	0.275	0.268	0.272	0.126
0	0.145	0.146	0.146

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Serum Intestinal Fatty Acid-Binding Protein and Calprotectin Concentrations to Assess Clinical Severity and Prognosis of Canine Parvovirus Enteritis CD Ay,Kafkas Universitesi Veteriner Fakultesi Dergisi,2023

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■ 常见问题解答

Would you provide the information:
1) antibody clonality _ are capture and detection antibodies monoclonal or polyclonal?
2) antibody's target immunogen _ to which immunogen have detection and capture antibodies of the kit been raised? was it of human or canine origin? was it a native or recombinant protein? was it a full length protein or a fragment?
3) proteins detected in the samples by the antibodies _ do the
antibodies of the kit detect only the S100A8/S100A9 heterocomplex or do they also detect S100A8 and S100A9 proteins separately (as monomers or homodimers)?
4) standard protein _ is the protein used as the analytical standard of the kit of canine or human origin? is it a native or recombinant protein? is it a full length protein or a fragment?

Thanks for your inquiry!CSB-EQ013485DO detects CALP(Calprotectin is a complex of the mammalian proteins S100A8 and S100A9)
The kit uses 2 antibodies, the Coated anti. and Detect anti. are both Goat polyclonal antibody, which are designed for the information of the links
https://www.ncbi.nlm.nih.gov/protein/XP_013970901.1 and https://www.ncbi.nlm.nih.gov/protein/NP_001139616.1 separately.
The immunogen is 293 cell-derived recombinant protein.
The standard is the purified crude product of the natural sample,but so sorry the specific source information can not be disclosed.

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Canine calprotectin (CALP) ELISA kit

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