Pig Trypsin ELISA Kit

说明书下载

中文名称：

猪胰蛋白酶(Trypsin)酶联免疫试剂盒
货号：

CSB-E15767p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪胰蛋白酶(Trypsin)酶联免疫检测试剂盒（货号：CSB-E15767p），适用于科研领域中对血清或血浆样本中胰蛋白酶水平的定量分析。胰蛋白酶是一种由胰腺分泌的关键消化酶，在蛋白质水解和肠道营养吸收中起重要作用，其表达水平的变化常与胰腺功能、代谢调控及消化系统疾病模型研究相关。CUSABIO试剂盒采用双抗体夹心法原理，检测灵敏度覆盖3.12 ng/mL至200 ng/mL的浓度区间，能够精准捕获目标蛋白并排除交叉反应干扰。实验操作包含预包被特异性抗体的酶标板、标准品及检测所需试剂，用户可通过标准曲线实现样本中胰蛋白酶含量的定量计算。该试剂盒可用于动物模型研究、胰腺外分泌功能评估、饲料营养代谢分析或酶活性相关机制探索等科研场景，为消化生理学、病理学及生物医药开发提供可靠的数据支持。
缩写：

Trypsin
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:5

Average %

Range %

83-97

1:10

Average %

Range %

80-90

1:20

Average %

Range %

84-99

1:40

Average %

Range %

88-100

回收率：

The recovery of pig trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-103
EDTA plasma (n=4)	99	94-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.192	2.131	2.162	2.029
100	1.769	1.834	1.802	1.669
50	1.289	1.319	1.304	1.171
25	0.824	0.867	0.846	0.713
12.5	0.543	0.577	0.560	0.427
6.25	0.372	0.337	0.355	0.222
3.12	0.288	0.259	0.274	0.141
0	0.137	0.129	0.133

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Pig Trypsin ELISA Kit

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