Canine trypsin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO犬胰蛋白酶（trypsin）定量检测酶联免疫试剂盒（货号：CSB-E12804c），采用双抗体夹心法原理设计，可精准检测血清、血浆及组织匀浆样本中39 ng/mL至2500 ng/mL浓度范围的胰蛋白酶含量。胰蛋白酶是胰腺分泌的关键消化酶，负责分解蛋白质为多肽，其水平变化常被用于评估犬类消化系统功能、胰腺外分泌状态及相关病理研究。试剂盒包含预包被特异性抗体的酶标板、标准品、检测抗体及显色底物等完整组分，操作流程经过优化，在3.5小时内即可完成检测。该产品适用于科研领域中对犬类胰腺生理机制、炎症反应或营养代谢相关课题的探索，例如通过检测不同实验条件下胰蛋白酶表达动态，解析其与疾病模型的关联性或评估药物干预效果。试剂盒支持多种生物样本类型，为研究者提供灵活的实验方案选择。
缩写：

trypsin
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

39 ng/mL-2500 ng/mL
灵敏度：

9.75 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	85-106
1:2	Average %	93
	Range %	85-105
1:4	Average %	95
	Range %	82-102
1:8	Average %	95
	Range %	84-110

回收率：

The recovery of canine trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	87-107
EDTA plasma (n=4)	97	86-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.