Mouse trypsin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠胰蛋白酶(trypsin)酶联免疫吸附检测试剂盒，采用双抗体夹心法定量检测血清、血浆、细胞培养上清液或组织匀浆样本中的胰蛋白酶浓度。胰蛋白酶是胰腺分泌的关键消化酶，在蛋白质分解代谢中起重要作用，其活性与胰腺功能、细胞培养中的细胞分离效率及炎症反应调控等过程密切相关。本试剂盒检测灵敏度为1.56 ng/mL，线性检测范围为1.56-100 ng/mL，可精准识别天然和重组形式的小鼠胰蛋白酶。适用于科研领域中对胰蛋白酶表达水平的定量分析，例如研究胰腺疾病模型中的酶活性变化、评估细胞培养体系中胰蛋白酶的释放规律，或分析组织样本在特定生理病理条件下的蛋白酶动态。试剂盒包含预包被特异性抗体的酶标板、标准品、检测抗体及显色系统，支持96孔板操作，实验全程约需4小时完成，兼容常规实验室设备进行吸光度测定。
缩写：

trypsin
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	103
	Range %	99-107
1:4	Average %	92
	Range %	87-96
1:8	Average %	98
	Range %	94-101

回收率：

The recovery of mouse trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	95	89-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.