Mouse procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Mouse procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

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中文名称：

小鼠Ⅰ型前胶原N端前肽(PⅠNP)酶联免疫试剂盒
货号：

CSB-E12775m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO小鼠Ⅰ型前胶原N端前肽（PⅠNP）酶联免疫检测试剂盒（货号：CSB-E12775m），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的PⅠNP含量，检测灵敏度为4.7 pg/mL，线性范围为4.7-300 pg/mL。PⅠNP是Ⅰ型胶原合成过程中的特异性代谢产物，可作为骨形成和纤维化进程的关键生物标志物，在骨代谢研究、纤维化疾病模型评估及药物干预效果分析中具有重要科研价值。试剂盒操作流程包含样本预处理（建议新鲜样本离心后取上清，组织样本需制备匀浆并经离心处理）、抗体孵育及显色反应，实验全程约需4.5小时，96孔板规格可满足多样本量需求。本产品提供预包被捕获抗体的酶标板、标准品、检测抗体及全套实验试剂，建议标准品和样本检测时设置复孔以保证数据可靠性，检测结果需在15分钟内使用酶标仪读取450nm吸光度值。适用于研究骨发育异常、骨质疏松模型、肝/肺纤维化机制探索等科研领域，为相关疾病模型的生物标志物分析提供可靠工具。
别名：

procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
缩写：

PⅠNP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.7 pg/mL-300 pg/mL
灵敏度：

1.17 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PINP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-98
1:200	Average %	95
	Range %	91-98
1:400	Average %	91
	Range %	85-96
1:800	Average %	94
	Range %	88-102

回收率：

The recovery of mouse PINP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-99
EDTA plasma (n=4)	89	82-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.413	2.313	2.363	2.261
150	1.598	1.498	1.548	1.446
75	0.940	0.890	0.915	0.813
37.5	0.489	0.479	0.484	0.382
18.75	0.316	0.306	0.311	0.209
9.4	0.207	0.205	0.206	0.104
4.7	0.145	0.142	0.144	0.042
0	0.103	0.101	0.102

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.