Rabbit procollagen Ⅰ N-terminal peptide(PⅠNP)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测兔Ⅰ型前胶原N端前肽（PⅠNP）的酶联免疫试剂盒，采用双抗体夹心法原理，适用于血清、血浆及多种组织匀浆样本的检测。PⅠNP是Ⅰ型胶原蛋白合成过程中的特异性代谢产物，作为骨形成的关键生物标志物，其浓度变化可反映骨代谢活性及成骨细胞功能状态，在骨组织发育、修复及代谢性疾病研究中具有重要价值。试剂盒检测灵敏度低至0.78 ng/mL，线性范围为0.78-50 ng/mL，通过预包被特异性捕获抗体和酶标记检测抗体的协同作用，实现对目标蛋白的精准定量分析。该产品可广泛应用于骨代谢机制研究、骨骼疾病动物模型评估、骨再生药物开发等科研领域，为骨生物学、骨组织工程及代谢性骨病的实验研究提供可靠数据支持。实验操作全程无需特殊设备，配套提供标准品及实验方案，满足实验室常规检测需求，仅限科学研究使用。
别名：

procollagen 1 N-terminal peptide(P1NP),_x000D_ procollagen I N-terminal peptide(PINP)
缩写：

PⅠNP
种属：

Oryctolagus cuniculus (Rabbit)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	103
	Range %	92-110
1:8	Average %	93
	Range %	84-98

回收率：

The recovery of rabbit PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	89	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.