Human procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人Ⅰ型前胶原N端前肽（PⅠNP）酶联免疫检测试剂盒（货号：CSB-E11226h），通过双抗体夹心法定量检测血清、血浆及组织匀浆样本中的PⅠNP浓度。PⅠNP是Ⅰ型胶原合成过程中的特异性代谢产物，作为骨形成的重要生物标志物，在骨代谢研究、骨质疏松机制探索及骨骼修复药物开发等领域具有重要科研价值。试剂盒检测灵敏度覆盖18.75至1200 pg/mL的线性范围，可精准捕捉样本中微量目标蛋白的动态变化。采用预包被高亲和力捕获抗体的96孔板，通过标准化的酶标二抗显色系统实现稳定检测，实验流程包含样本预处理、孵育、洗涤和读数步骤，配备标准品和对照品确保检测重复性。适用于骨组织工程研究、骨骼疾病动物模型评估、骨代谢相关分子机制解析等科研场景，为骨代谢研究、抗骨质疏松药物筛选及骨再生材料开发提供可靠工具。
别名：

procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
缩写：

PⅠNP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

18.75 pg/mL-1200 pg/mL
灵敏度：

4.68 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PⅠNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	97
	Range %	89-102
1:200	Average %	90
	Range %	83-97
1:400	Average %	99
	Range %	94-105
1:800	Average %	96
	Range %	90-102

回收率：

The recovery of human PⅠNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-99
EDTA plasma (n=4)	101	95-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.