Mouse Lysozyme,LZM ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠溶菌酶（LZM）酶联免疫试剂盒（货号：CSB-E11329m）基于双抗体夹心法原理开发，可定量检测血清、血浆及组织匀浆中的LZM蛋白浓度。溶菌酶是一种广泛分布于生物体液中的水解酶，通过分解细菌细胞壁的肽聚糖成分发挥先天免疫防御功能，其表达水平与免疫调节、炎症反应及组织修复等过程密切相关。本试剂盒检测灵敏度为0.195 ng/mL，线性检测范围为0.78-50 ng/mL，适用于多种生物样本的精准检测。产品采用96孔板规格，包含预包被捕获抗体、生物素标记检测抗体、标准品、HRP标记链霉亲和素及TMB显色液等核心组分，操作流程包含标准品梯度稀释、样本孵育、显色反应及终止检测步骤，实验全程约需4.5小时。试剂盒组分需在-20°C避光保存，有效期12个月。该产品可广泛应用于小鼠感染性疾病模型、免疫系统功能评估、炎症机制研究以及药物干预实验中溶菌酶动态变化的检测，为科研人员探究先天免疫应答机制提供可靠工具。
缩写：

LZM
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	89-98
1:200	Average %	97
	Range %	94-101
1:400	Average %	98
	Range %	93-103
1:800	Average %	93
	Range %	87-100

回收率：

The recovery of mouse LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-100
EDTA plasma (n=4)	91	85-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.