Bovine Lysozyme (LZM) ELISA Kit

产品详情

产品描述：

牛溶菌酶(LZM)酶联免疫试剂盒（CSB-E15829B）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LZM含量。牛溶菌酶（LZM）是一种具有抗菌、抗炎、抗病毒和免疫调节功能的碱性蛋白，其作用机制主要是通过裂解细菌细胞壁的肽聚糖结构，以及与病毒蛋白结合使其失活。近年来，研究者通过基因工程和转基因技术，提高了LZM的表达量和活性，拓展了其在食品、医药和生物材料等领域的应用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，支持科研人员探究反刍动物免疫调节机制、病原体防御反应等方向，例如在畜牧业中评估饲养环境对牛群免疫健康的影响，或基础研究中解析LZM在组织局部抗感染过程中的动态表达规律，为动物模型构建及生物标志物研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

LYZ1Lysozyme C ELISA Kit; EC 3.2.1.17 ELISA Kit; 1,4-beta-N-acetylmuramidase C ELISA Kit
缩写：

LZM
Uniprot No.：

P04421
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	101
	Range %	96-105
1:400	Average %	90
	Range %	85-95
1:800	Average %	106
	Range %	100-110
1:1600	Average %	104
	Range %	99-109

回收率：

The recovery of bovine LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-98
EDTA plasma (n=4)	100	94-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
蛋白家族：

Glycosyl hydrolase 22 family
组织特异性：

Stomach-specific.
数据库链接：

KEGG: bta:280849

UniGene: Bt.29367