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Fish lysozyme (renal amyloidosis) (LZM) ELISA kit

  • 中文名称:
    鱼溶菌酶(肾淀粉样变)(LZM)酶联免疫试剂盒
  • 货号:
    CSB-E17296Fh
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

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  • 产品描述:
    鱼溶菌酶(肾淀粉样变)(LZM)酶联免疫试剂盒(CSB-E17296Fh)为竞争法ELISA试剂盒,定量检测血清、血浆样本中的LZM含量。LZM即溶菌酶,是一种能水解致病菌中黏多糖的碱性酶。广泛存在于动植物及微生物中,具有抗菌、抗病毒等特性。研究机制主要是通过破坏细菌细胞壁的肽聚糖,使细菌溶解死亡,在医药、食品等领域有潜在应用价值。试剂盒检测范围为3.12 ng/mL-200 ng/mL,该产品主要应用于水产养殖中的鱼类健康监测、免疫相关疾病机制研究以及水质污染对鱼类生理影响的评估等领域,为实验室开展鱼类病理学、毒理学及免疫学研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    LZM
  • 种属:
    Fish
  • 样本类型:
    serum, plasma
  • 检测范围:
    3.12 ng/mL-200 ng/mL
  • 灵敏度:
    1.56 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 93  
    Range % 85-100  
    1:40 Average % 91  
    Range % 86-99  
    1:80 Average % 95  
    Range % 90-103  
    1:160 Average % 95  
    Range % 90-98  
  • 回收率:
    The recovery of fish LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 87-94  
    EDTA plasma (n=4) 93 85-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    200 0.088 0.090 0.089    
    100 0.206 0.194 0.200    
    50 0.307 0.300 0.304    
    25 0.418 0.423 0.421    
    12.5 0.627 0.605 0.616    
    6.25 1.045 1.031 1.038    
    3.12 1.648 1.524 1.586    
    0 2.685 2.657 2.671    
  • 数据处理:
  • 货期:
    3-5 working days

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