Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

Mouse Angiotensin 1-7(Ang1-7)ELISA Kit

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中文名称：

小鼠血管紧张素1-7（Ang1-7）酶联免疫试剂盒
货号：

CSB-E13763m
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专用于科研的小鼠血管紧张素1-7（Ang1-7）定量检测试剂盒（货号：CSB-E13763m），基于双抗体夹心酶联免疫原理，可精准检测血清、血浆、细胞培养上清及组织匀浆中的Ang1-7含量，检测灵敏度达6.25 pg/mL，线性范围为6.25-400 pg/mL。Ang1-7作为肾素-血管紧张素系统（RAS）的关键活性肽，在心血管调节、抗纤维化及炎症反应中发挥重要作用，其水平变化常被用于心血管疾病模型研究、代谢综合征机制探索及药物干预效果评估。试剂盒包含预包被捕获抗体的96孔板、梯度标准品、生物素标记检测抗体及显色底物，实验全程仅需3.5小时，操作流程标准化，支持多样本批量检测。适用于实验室对小鼠疾病模型中Ang1-7的动态监测，如高血压、心肌肥厚、肾脏损伤等研究的生物标志物分析，或体外药物筛选实验中细胞因子分泌水平的定量评估。实验需注意样本预处理，避免反复冻融，血浆样本建议使用EDTA抗凝管采集，组织样本需按比例匀浆后离心取上清检测。
缩写：

Ang1-7
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	93
	Range %	87-99
1:4	Average %	95
	Range %	89-102
1:8	Average %	95
	Range %	87-98

回收率：

The recovery of mouse Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-98
EDTA plasma (n=4)	95	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.745	2.721	2.733	2.597
200	2.316	2.245	2.281	2.145
100	1.923	1.898	1.911	1.775
50	1.467	1.366	1.417	1.281
25	0.796	0.788	0.792	0.656
12.5	0.458	0.447	0.453	0.317
6.25	0.258	0.242	0.250	0.114
0	0.138	0.134	0.136

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Human placental mesenchymal stem cells transplantation repairs the alveolar epithelial barrier to alleviate lipopolysaccharides-induced acute lung injury W Yu, Y Lv, R Xuan, P Han, H Xu, X Ma,Biochemical Pharmacology,2024
A53T α-synuclein mutation increases susceptibility to postoperative delayed neurocognitive recovery via hippocampal Ang-(1–7)/MasR axis J Hong,Biochemical pharmacology,2024
Mas receptor activation facilitates innate hematoma resolution and neurological recovery after hemorrhagic stroke in mice X Deng,Journal of neuroinflammation,2024
Potential therapeutic implications of calcitriol administration and weight reduction on CD4 T cell dysregulation and renin angiotensin system-associated acute lung injury in septic obese mice CL Yeh,Biomedicine & pharmacotherapy,2023
ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway F Chen,Journal of translational medicine,2023
Recombinant human angiotensin-converting enzyme 2 plays a protective role in mice with sepsis-induced cardiac dysfunction through multiple signaling pathways dependent on converting angiotensin II to angiotensin 1–7 C Wu,Annals of Translational Medicine,2023
Renal angiotensin I-converting enzyme-deficient mice are protected against aristolochic acid nephropathy A Juretzko,Pflugers Archiv,2022
Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entry Zilun L,Cell Metabolism,2022
ACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury Tomokazu Yamaguchi,nature communications,2021
Ambient fine particulate matter induced the elevation of blood pressure through ACE2/Ang(1–7) pathway: The evidence from urine metabolites X Du,Ecotoxicology and Environmental Safety,2020
B38-CAP is a bacteria-derived ACE2-like enzyme that suppresses hypertension and cardiac dysfunction T Minato,Nature Communications,2020
Ginsenoside Rg3 Attenuates Angiotensin II-Mediated Renal Injury in Rats and Mice by Upregulating Angiotensin-Converting Enzyme 2 in the Renal Tissue Liu H, et al,Evidence-Based Complementary and Alternative Medicine,2019
Effects of exendin-4 on the intrarenal renin-angiotensin system and interstitial fibrosis in unilateral ureteral obstruction mice: Exendin-4 and unilateral ureteral obstruction Ying Le.et al,SAGE Journals,2016
The effects of different angiotensin II type 1 receptor blockers on the regulation of the ACE-AngII-AT1 and ACE2-Ang(1-7)-Mas axes in pressure overload-induced cardiac remodeling in male mice. Wang X.et al,J Mol Cell Cardiol.,2016
Control of Adipogenesis by the Autocrine Interplays between Angiotensin (1-7)/Mas receptor and Angiotensin II/AT1 receptor Signaling Pathways Than A et al,J Biol Chem,2013

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.