Dog angiotensin 1-7 (Ang1-7) ELISA kit

产品详情

产品描述：

CUSABIO犬血管紧张素1-7（Ang1-7）酶联免疫检测试剂盒（货号CSB-EQ027505DO），专为科研领域设计，适用于定量分析血清、血浆、组织匀浆及细胞培养上清液中的Ang1-7水平。Ang1-7作为肾素-血管紧张素系统（RAS）的关键活性肽，在调节血压平衡、抑制炎症反应及改善器官纤维化中发挥重要作用，是心血管与代谢性疾病机制研究的重要靶标。该试剂盒基于双抗体夹心法原理，检测灵敏度高，可精准覆盖6.25-400 pg/mL的浓度范围，最低检测限达1.56 pg/mL，满足多样本类型的低丰度靶标检测需求。其预包被高特异性抗体与优化的反应体系，可在4小时内完成检测流程，操作便捷且重复性优异。研究者可将其应用于犬类心血管疾病模型、肾脏功能研究、代谢综合征机制探索，以及药物干预或基因调控对Ang1-7通路影响的体外实验分析，为深入解析RAS系统在病理生理过程中的作用提供可靠工具。
缩写：

Ang1-7
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-92
1:2	Average %	100
	Range %	97-103
1:4	Average %	86
	Range %	83-89
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of dog Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	91	85-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.