Human Angiotensin 1-7(Ang1-7)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人血管紧张素1-7（Ang1-7）酶联免疫检测试剂盒（货号：CSB-E14242h），采用双抗体夹心法原理实现定量检测，适用于血清、血浆及细胞培养上清液样本中Ang1-7的精准分析。Ang1-7作为肾素-血管紧张素系统（RAS）的关键活性肽，在调节血压平衡、抗纤维化及心血管保护中发挥重要作用。试剂盒检测灵敏度达7.8 pg/mL，线性范围为7.8-500 pg/mL，可在3.5小时内完成检测流程，预包被96孔板提供便捷操作，包含标准品、生物素标记检测抗体、链霉亲和素-HRP及TMB显色系统等核心组分。该试剂盒适用于心血管疾病机制研究、药物干预效果评估、细胞模型中Ang1-7分泌水平检测等科研领域，尤其适合低丰度样本的定量分析。标准曲线经严格验证，实验步骤明确规范，配套终止液可确保显色反应稳定性，为体外实验提供可靠的Ang1-7浓度检测方案。
缩写：

Ang1-7
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, cell culture supernates
检测范围：

7.8 pg/mL-500 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Ang1-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-105
1:2	Average %	96
	Range %	85-109
1:4	Average %	105
	Range %	92-114
1:8	Average %	95
	Range %	82-110

回收率：

The recovery of human Ang1-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-110
EDTA plasma (n=4)	99	90-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.