Human activated protein C,APC ELISA Kit

Human activated protein C,APC ELISA Kit

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中文名称：

人活化蛋白C(APC)酶联免疫试剂盒
货号：

CSB-E09909h
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO人活化蛋白C（APC）酶联免疫检测试剂盒（货号：CSB-E09909h），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中APC的浓度。APC是凝血系统关键调节因子，通过灭活凝血因子Va和VIIIa发挥抗凝作用，同时参与调节炎症反应和细胞凋亡，在血栓性疾病、败血症及肿瘤微环境等研究领域具有重要价值。试剂盒检测灵敏度高，线性范围为1.56-100 pg/mL，可精准捕捉痕量APC水平变化，适用于凝血调控机制、炎症相关疾病模型或药物干预等基础研究。实验操作采用预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，配合标准品建立标准曲线进行定量分析。样本兼容性强，支持血清（避免溶血）、肝素/EDTA抗凝血浆及组织匀浆（需离心去除杂质）等多种类型，建议实验前通过预稀释优化样本浓度至检测范围内。本试剂盒提供完整组分包括标准品、检测抗体、显色底物及终止液，4℃和-20℃分装保存，确保批次稳定性和实验结果可靠性。
别名：

N/A
缩写：

APC
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	87-96
1:200	Average %	94
	Range %	90-97
1:400	Average %	97
	Range %	94-103
1:800	Average %	94
	Range %	90-98

回收率：

The recovery of human APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	87	84-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.755	2.990	2.873	2.754
50	2.065	2.201	2.133	2.014
25	1.359	1.288	1.324	1.205
12.5	0.853	0.816	0.835	0.716
6.25	0.470	0.473	0.472	0.353
3.12	0.325	0.302	0.314	0.195
1.56	0.184	0.192	0.188	0.069
0	0.115	0.122	0.119

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Elucidating mechanisms of genetic cross-disease associations at the PROCR vascular disease locus David Stacey,Nature Communications,2022
Evaluation of trauma‐induced coagulopathy in the fibrinogen in the initial resuscitation of severe trauma trial HT Peng,Transfusion,2021
Alterations of anticoagulant proteins and soluble endothelial protein C receptor in thalassemia patients of Chinese origin YulanHuang.et al,Thrombosis Research,2018
Coagulation Factor Hyperfunction After Subarachnoid Hemorrhage Induces Deep Venous Thrombosis Wang Miao.et al,World Neurosurgery,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.