Pig activated protein C(APC) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用竞争法酶联免疫吸附原理，适用于定量检测猪血清、血浆及组织匀浆中的活化蛋白C（APC）含量。APC是一种由凝血酶-血栓调节蛋白复合物激活的丝氨酸蛋白酶，在抗凝血、抗炎及细胞保护等生理过程中发挥关键作用，其水平变化常与凝血功能异常或炎症反应相关疾病的研究密切相关。该试剂盒检测范围为40 pg/mL至8000 pg/mL，能够精准覆盖猪体内APC的生理及病理浓度范围，通过竞争结合反应实现定量分析：样本中的APC与标记物竞争结合预包被抗体，显色信号强度与APC浓度呈负相关。该产品适用于科研领域，如猪凝血机制研究、炎症相关疾病模型（如败血症或血栓性疾病）的建立与评估，以及抗凝药物或生物制剂开发过程中药效动力学研究。其兼容多种样本类型的特点，可支持不同实验设计需求，例如通过组织匀浆分析特定器官（如肝脏或血管内皮）中APC的局部表达水平，为深入探究APC在病理生理过程中的调控机制提供可靠工具。
别名：

N/A
缩写：

APC
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

40 pg/mL-8000 pg/mL
灵敏度：

20 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	95
	Range %	89-101
1:4	Average %	94
	Range %	91-99
1:8	Average %	96
	Range %	93-99

回收率：

The recovery of pig APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	91-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.