Mouse activated protein C,APC ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于科研领域的小鼠活化蛋白C（APC）酶联免疫试剂盒（货号：CSB-E09914m），基于夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中APC水平的精准分析。活化蛋白C作为重要的丝氨酸蛋白酶，在调节凝血系统、抗炎及细胞保护等生理过程中发挥关键作用，其水平变化与血栓形成、炎症反应及内皮功能异常等研究密切相关。试剂盒检测灵敏度高，线性范围覆盖62.5 pg/mL至4000 pg/mL，可满足不同浓度样本的检测需求。实验操作采用预包被特异性抗体的微孔板，通过双抗体夹心法捕获目标蛋白，结合酶标抗体显色系统实现定量分析，确保数据的稳定性和重复性。该试剂盒广泛应用于基础医学研究，如凝血/纤溶系统调控机制探索、炎症相关疾病动物模型评估、药物干预对APC通路的影响研究等，尤其适用于从多类型样本中系统性分析APC的动态变化，为心血管疾病、败血症等领域的科研工作提供可靠工具。
别名：

N/A
缩写：

APC
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse APC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	90
	Range %	86-93
1:4	Average %	94
	Range %	90-97
1:8	Average %	99
	Range %	95-103

回收率：

The recovery of mouse APC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	95	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.