Human Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO人Ⅰ型前胶原羧基端肽（PⅠCP）酶联免疫检测试剂盒（货号：CSB-E08079h），采用双抗体夹心法定量分析血清、血浆或尿液样本中的PⅠCP含量。PⅠCP是Ⅰ型胶原蛋白合成过程中的特异性代谢产物，其浓度变化可反映骨代谢活性及结缔组织重塑状态，常用于骨转化研究、纤维化疾病模型评估及组织修复机制探索等科研领域。试剂盒检测范围宽达62.5-4000 pg/mL，可满足不同浓度样本的检测需求，标准曲线灵敏度最低可测至62.5 pg/mL。实验采用预包被高特异性抗体，通过优化反应体系实现精准定量，操作流程包含样本预处理、孵育、洗涤和显色步骤，配套提供标准品与实验缓冲液。该试剂盒适用于骨代谢相关细胞实验、动物模型研究，以及肝纤维化、肺纤维化等疾病机制研究，尿液样本的兼容性为无创性研究提供了便利。本产品仅用于科学研究，实验结果需结合其他指标综合分析。
别名：

Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
缩写：

PⅠCP
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, urine
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	99-101
1:200	Average %	91
	Range %	85-97
1:400	Average %	87
	Range %	82-94
1:800	Average %	95
	Range %	90-100

回收率：

The recovery of human PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	81-93
EDTA plasma (n=4)	100	93-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.