Mouse Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠Ⅰ型前胶原羧基端肽(PⅠCP)酶联免疫检测试剂盒（货号：CSB-E08080m），主要用于定量检测小鼠血清、血浆样本中PⅠCP的含量。PⅠCP是Ⅰ型胶原蛋白合成过程中的特异性代谢产物，其水平可反映Ⅰ型胶原的合成速率，在骨代谢调控、纤维化疾病机制研究等领域具有重要科研价值。CUSABIO试剂盒采用双抗体夹心法原理，检测范围为31.25-2000 pg/mL，能够精准检测微量目标分子，实验过程包含预包被抗体、生物素标记检测抗体及链霉亲和素-HRP系统，具有较高的灵敏度和稳定性。适用于骨代谢相关疾病模型（如骨质疏松、骨发育异常）的实验研究，也可用于肝纤维化、肺纤维化等组织修复与纤维化进程的机制探讨，为体外研究Ⅰ型胶原代谢通路提供可靠的检测工具。试剂盒严格排除种属交叉反应，操作流程标准化，满足实验室对生物样本中PⅠCP的定量分析需求。
别名：

Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
缩写：

PⅠCP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	90-99
1:200	Average %	91
	Range %	85-96
1:400	Average %	87
	Range %	82-93
1:800	Average %	96
	Range %	92-102

回收率：

The recovery of mouse PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-95
EDTA plasma (n=4)	92	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.