Rat Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠Ⅰ型前胶原羧基端肽(PⅠCP)酶联免疫检测试剂盒（货号：CSB-E08081r），基于双抗体夹心法原理实现定量检测，适用于血清、血浆样本中PⅠCP的精确分析。PⅠCP是Ⅰ型胶原蛋白合成过程中的特异性裂解产物，其浓度变化可反映机体胶原代谢状态，尤其在骨基质形成和结缔组织重塑过程中具有重要生物学意义。试剂盒检测范围为3.12-200 ng/mL，能够满足不同浓度样本的检测需求，通过优化的抗体配对体系确保检测的特异性与准确性，实验流程包含标准品预包被板、生物素标记检测抗体及链霉亲和素-HRP反应系统。该试剂盒可应用于骨骼发育研究、纤维化疾病模型评估、骨代谢相关药物筛选等科研领域，为探究骨形成活性、组织修复机制及病理状态下的胶原合成动态提供可靠工具。实验需自备酶标仪、移液器等基础设备，建议通过标准曲线绘制实现样本浓度的精准计算。
别名：

Carboxyterminal propeptide of type 1 procollagen,P1CP,_x000D_ Carboxyterminal propeptide of type I procollagen,PICP
缩写：

PⅠCP
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-96
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	88-97
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of rat PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-89
EDTA plasma (n=4)	90	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.