Human activated coagulation factor X(FXa) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO定量检测人活化凝血因子X（FXa）的ELISA试剂盒（货号：CSB-E12696h），采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆样本中0.312-20 ng/mL浓度范围内的FXa水平。FXa是凝血级联反应中的关键蛋白酶，通过切割凝血酶原生成凝血酶，在止血与血栓形成中发挥核心作用，其活性监测对凝血机制研究及抗凝药物开发具有重要意义。试剂盒包含预包被特异性抗体的96孔板，通过标准孵育流程捕获靶蛋白，结合HRP标记检测抗体与TMB显色系统实现定量分析，操作步骤包含样本预处理、孵育、洗涤及显色，实验全程约4-5小时。该产品适用于凝血相关分子机制研究、血栓性疾病模型分析、抗凝剂药效评估等科研领域，实验数据稳定可靠，配套提供标准品、试剂及操作指南，满足体外实验需求。
缩写：

FXa
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-100
1:2	Average %	92
	Range %	87-96
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	83-96

回收率：

The recovery of human FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-102
EDTA plasma (n=4)	96	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.