Rat activated coagulation factor X(FXa) ELISA Kit

产品详情

产品描述：

大鼠活化凝血因子X(FXa)酶联免疫试剂盒（CSB-EQ027251RA）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FXa含量。FXa即凝血因子Xa，是凝血级联反应中的关键靶点。它在凝血过程中处于内、外源凝血途径交汇点，激活后促使凝血酶原转化为凝血酶。对其研究机制主要聚焦于开发抑制剂，以抑制凝血过程，用于血栓性疾病防治。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，支持实验室开展凝血机制研究、抗凝药物筛选、病理模型评估等方向实验，多样本兼容性设计尤其适合系统性研究凝血因子在组织微环境中的动态变化，为探索血栓性疾病、术后凝血异常等课题提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

FXa
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FXa in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	102
	Range %	98-109
1:400	Average %	90
	Range %	86-94
1:800	Average %	97
	Range %	92-101

回收率：

The recovery of rat FXa spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	89	85-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.