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Rat Interleukin 23,IL-23 ELISA Kit

  • 中文名称:
    大鼠白介素23(IL-23)酶联免疫试剂盒
  • 货号:
    CSB-E08462r
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:
    大鼠白介素23(IL-23)酶联免疫试剂盒(CSB-E08462r)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL-23含量。IL-23是一种细胞因子,在免疫系统中发挥重要作用。其与炎症、自身免疫性疾病密切相关,如银屑病、炎症性肠病等。研究机制显示,它可激活特定的T细胞亚群,促进炎症因子的产生,引发免疫反应,目前是药物研发的热门靶点。试剂盒检测范围为3.12 pg/mL-200 pg/mL,适用于体外炎症模型研究、免疫通路调控实验或药物干预效果评估等科研领域,为探索IL - 23在神经免疫、肠道屏障功能或慢性炎症反应中的分子机制提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    N/A
  • 缩写:
    IL-23
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    3.12 pg/mL-200 pg/mL
  • 灵敏度:
    0.78 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %89-94
    1:2Average %104
    Range %100-107
    1:4Average %92
    Range %87-95
  • 回收率:
    The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-95
    EDTA plasma (n=4)9894-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.147 2.112 2.130 1.998
    1001.851 1.776 1.814 1.682
    501.308 1.288 1.298 1.166
    250.903 0.898 0.901 0.769
    12.50.621 0.614 0.618 0.486
    6.250.375 0.359 0.367 0.235
    3.120.236 0.224 0.230 0.098
    00.134 0.130 0.132  
  • 数据处理:
  • 货期:
    3-5 working days

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相关问答

 常见问题解答
Q:

I am interested in measuring cytokines in patients affected by autoimmune thyroid diseases. For instance, i found the kit
(Human Interleukin 23 (IL-23) ELISA Kit,Catalog Number. CSB-E08461h) of your company,
Can this kit be used for the same purpose in lab animals (mouse, rats), if so, we are also interested in measuring IL-21, IL-22, IL-17A, IL-17F, all relevant kits produced by the same company, can they also be used for the same intention in lab animals??

A:
Thanks for your inquiry. For these cytokines IL-23, IL-21, IL-22, IL-17A, IL-17F,detection of cross-species is not advised,as it will be affected by the characteristics of antibodies and the matrix difference.
Regarding your research in measuring cytokines in patients affected by autoimmune thyroid diseases,we suggest you use human kits.While regarding your early laboratory research on lab animals,we suggest you use the corresponding lab animals kits.
Human kits:
#CSB-E08461h,Human Interleukin 23,IL-23 ELISA Kit
#CSB-E11707h,Human Interleukin 21,IL-21 ELISA Kit
#CSB-E13418h,Human Interleukin 22(IL-22) ELISA KIT
#CSB-E12819hHuman Interleukin 17A (IL-17A/IL-17) ELISA Kit
#CSB-E15916h,Human Interleukin17F (IL17F) ELISA Kit
Mouse kits:
#CSB-E08463m,Mouse Interleukin 23,IL-23 ELISA Kit
#CSB-E13162m,Mouse Interleukin 21(IL-21) ELISA Kit
#CSB-E13513m,Mouse Interleukin 22(IL-22) ELISA Kit
#CSB-E04608m,Mouse Interleukin 17,IL-17 ELISA Kit
#CSB-EL011601MO,Mouse Interleukin-17F(IL17F) ELISA kit
Rat kits:
#CSB-E08462r,Rat Interleukin 23,IL-23 ELISA Kit
#CSB-E11843r,Rat Interleukin 21,IL-21 ELISA Kit
#CSB-E14918r,Rat Interleukin 22(IL-22) ELISA Kit
#CSB-E07451r,Rat Interleukin 17,IL-17 ELISA KIT
#CSB-EL011601RA,Rat Interleukin-17F(IL17F) ELISA kit

If you are interested to order any one of them,we will foward your request to local distributor.Well,could you advise which coutry are you from?Thank you very much!