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Mouse malonyl coenzyme A ELISA kit

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小鼠丙二酸单酰辅酶A(malonyl CoA)酶联免疫试剂盒（CSB-E12896m）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的malonyl CoA含量。Malonyl CoA是一种重要的代谢中间产物。它参与脂肪酸合成与氧化的调控，在能量代谢平衡中起关键作用。研究主要聚焦其在调控肉碱棕榈酰转移酶1活性以调节脂肪酸进入线粒体氧化的机制，与代谢疾病如糖尿病、肥胖等的关联也是热点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，广泛应用于代谢性疾病机制研究，如糖尿病、肥胖症模型中的脂肪酸合成与分解动态分析，也可用于肝脏、肌肉等代谢活跃组织的能量代谢调控研究，为探索营养干预、基因调控对脂质代谢的影响提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

malonyl CoA
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	84-996
1:2000	Average %	95
	Range %	89-99
1:4000	Average %	99
	Range %	95-105
1:8000	Average %	98
	Range %	90-106

回收率：

The recovery of mouse malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-96
EDTA plasma (n=4)	95	87-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.214	2.298	2.256	2.159
5	1.791	1.765	1.778	1.681
2.5	1.224	1.268	1.246	1.149
1.25	0.842	0.812	0.827	0.730
0.625	0.572	0.547	0.560	0.463
0.312	0.381	0.365	0.373	0.276
0.156	0.201	0.209	0.205	0.108
0	0.096	0.098	0.097

本试剂盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Mouse malonyl CoA antibody.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled malonyl CoA antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.