Rat malonyl coenzyme A ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠丙二酸单酰辅酶A（malonyl CoA）酶联免疫检测试剂盒（货号：CSB-E12893r），适用于定量检测血清、血浆及组织匀浆样本中1.25 ng/mL至20 ng/mL浓度范围的malonyl CoA含量。malonyl CoA是脂肪酸代谢的关键调节分子，通过抑制肉碱棕榈酰转移酶1（CPT1）调控脂肪酸氧化与合成的平衡，在能量稳态、胰岛素敏感性及脂质代谢相关疾病研究中具有重要价值。CUSABIO试剂盒采用竞争性酶联免疫吸附测定技术，通过特异性抗体与样本中目标分子及酶标抗原的竞争结合反应实现定量分析，实验操作标准化且灵敏度高，可满足能量代谢调控机制、肥胖及糖尿病模型研究、心血管疾病相关脂代谢异常探索等科研需求。试剂盒兼容多种生物样本类型，为实验室开展脂肪酸代谢通路研究、药物干预效果评估或营养代谢调控实验提供可靠工具，助力科研人员高效获取精准数据。
缩写：

malonyl CoA
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 ng/mL-20 ng/mL
灵敏度：

0.625 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	92-103
1:200	Average %	102
	Range %	100-104
1:400	Average %	93
	Range %	88-97
1:800	Average %	89
	Range %	84-93

回收率：

The recovery of rat malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	84	80-88

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.