Human 5-Hydroxytryptamine,5HT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人5羟色胺(5-HT)酶联免疫试剂盒（CSB-E08363h）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的5HT含量。5HT即血清素，是一种重要的神经递质。其在调节情绪、睡眠、食欲等方面发挥关键作用。研究机制上，主要通过与不同受体结合，影响神经信号传导来调节生理和心理功能，对其研究有助于揭示相关疾病发病机制及开发新药。试剂盒检测范围为4 ng/mL-800 ng/mL，特别适用于神经递质代谢研究、精神疾病动物模型分析、药理学实验中的药效评估等科研领域，例如探究抑郁症或肠易激综合征模型中5-HT的动态变化，或筛选影响5-HT合成/代谢的化合物。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

5HT
种属：

Homo sapiens (Human)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4 ng/mL-800 ng/mL
灵敏度：

2 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-102
1:2	Average %	94
	Range %	91-98
1:4	Average %	85
	Range %	80-89
1:8	Average %	95
	Range %	89-99

回收率：

The recovery of human 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	89	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.