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Mouse 5-Hydroxytryptamine,5HT ELISA Kit

  • 中文名称:
    小鼠5羟色胺(5-HT)酶联免疫试剂盒
  • 货号:
    CSB-E08365m
  • 规格:
    96T/48T
  • 价格:
    ¥3800/¥2500
  • 其他:

产品详情

  • 产品描述:
    本试剂盒(货号CSB-E08365m)采用竞争法酶联免疫吸附原理,专为定量检测小鼠血清、血浆及组织匀浆中的5-羟色胺(5-HT)设计。5-HT作为关键单胺类神经递质,广泛参与情绪调节、睡眠周期、胃肠功能及疼痛感知等生理过程,其浓度变化与神经退行性疾病、抑郁症及肠道功能紊乱等研究密切相关。试剂盒检测灵敏度达250 pg/mL,线性范围覆盖250-4000 pg/mL,通过预包被特异性抗体实现高精度检测:样本中的内源性5-HT与生物素标记的抗原竞争结合抗体,结合HRP-链霉亲和素催化底物显色,显色强度与目标物浓度呈负相关。该产品适用于神经科学研究中的递质动态分析、药理学实验的干预效果评估、行为学模型验证以及代谢相关疾病动物模型的机制探索,操作全程无需特殊设备,可在常规实验室条件下完成,配套提供标准品、预混试剂及详细操作指南,确保科研数据的可靠性与重复性。
  • 缩写:
    5HT
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    250 pg/mL-4000 pg/mL
  • 灵敏度:
    125 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse 5HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 90-89  
    1:200 Average % 90  
    Range % 86-94  
    1:400 Average % 84  
    Range % 80-88  
    1:800 Average % 97  
    Range % 92-101  
  • 回收率:
    The recovery of mouse 5HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-99  
    EDTA plasma (n=4) 89 83-96  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    4000 0.130 0.120 0.125    
    2000 0.243 0.241 0.242    
    1000 0.452 0.419 0.436    
    500 0.792 0.831 0.812    
    250 1.068 1.120 1.094    
    0 2.151 2.026 2.089    
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型:细胞培养上清液

样品信息:小鼠

稀释比:其他 1:2

产品评价: 我用检测PC12细胞上清液,标准曲线的OD值为y = -1.0317x + 2.6063,R² = 0.9866 样本的OD值为0.1084~202402 快速便捷,非常好用

By 廖老师