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Monkey Vascular Endothelial cell Growth Factor(VEGF)ELISA Kit

  • 中文名称:
    猴子血管内皮细胞生长因子(VEGF)酶联免疫试剂盒
  • 货号:
    CSB-E16502Mk
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    CUSABIO专用于检测猴血清、血浆、细胞培养上清及细胞裂解液中血管内皮细胞生长因子(VEGF)含量的酶联免疫试剂盒,采用双抗体夹心法定量分析,检测灵敏度高且特异性强。VEGF作为调控血管生成的核心因子,在组织修复、肿瘤生长及炎症反应等科研领域具有重要研究价值。试剂盒检测范围覆盖125-8000 pg/mL,可精准检测不同浓度梯度的VEGF表达水平,适用于体外培养的灵长类细胞模型中血管生成机制研究、药物干预实验的效应评估或相关疾病动物模型样本分析。该试剂盒提供预包被96孔板及标准化试剂,操作流程经优化可实现4小时内完成检测,样本兼容性广泛,支持血清/血浆样本直接检测(需避免反复冻融),细胞培养上清建议离心去除颗粒物后使用。其稳定的标准曲线性能(R²≥0.99)和重复性验证数据,可为心血管疾病研究、肿瘤微环境分析等基础科研提供可靠定量依据,满足实验室对灵长类样本中VEGF动态监测的需求。
  • 缩写:
    VEGF
  • 种属:
    Monkey
  • 样本类型:
    serum, plasma, cell culture supernates, cell lysates
  • 检测范围:
    125 pg/mL-8000 pg/mL
  • 灵敏度:
    31.25 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.422 2.276 2.349 2.206
    40002.048 1.954 2.001 1.858
    20001.443 1.511 1.477 1.334
    10000.746 0.777 0.762 0.619
    5000.472 0.467 0.470 0.327
    2500.358 0.366 0.362 0.219
    1250.241 0.234 0.238 0.095
    00.145 0.141 0.143
  • 数据处理:
  • 货期:
    3-5 working days

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