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Rabbit Vascular Endothelial cell Growth Factor,VEGF ELISA Kit

  • 中文名称:
    兔血管内皮细胞生长因子(VEGF)酶联免疫试剂盒
  • 货号:
    CSB-E08524Rb
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    CUSABIO兔血管内皮细胞生长因子(VEGF)酶联免疫检测试剂盒(货号:CSB-E08524Rb),采用双抗体夹心法原理,可定量检测血清、血浆及组织匀浆样本中VEGF的浓度,检测范围为1.56 pg/mL至100 pg/mL。VEGF是一种调控血管生成和内皮细胞增殖的关键蛋白,广泛参与组织修复、炎症反应及病理状态下的血管新生过程。该试剂盒通过高特异性抗体对靶标分子进行精准捕获与信号放大,适用于科研场景下探究血管生成相关机制、评估疾病模型中VEGF的动态变化,或用于药物研发中靶向分子效应的体外检测。实验操作简便,支持多样本类型分析,可为心血管疾病、肿瘤微环境等领域的基础研究提供可靠数据支持。本产品仅供科学研究使用。
  • 缩写:
    VEGF
  • 种属:
    Oryctolagus cuniculus (Rabbit)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.56 pg/mL-100 pg/mL
  • 灵敏度:
    0.39 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %81-95
    1:2Average %100
    Range %91-106
    1:4Average %94
    Range %92-105
    1:8Average %90
    Range %86-98
  • 回收率:
    The recovery of rabbit VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9791-100
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.538 2.540 2.539 2.403
    502.012 2.022 2.017 1.881
    251.137 1.130 1.134 0.998
    12.50.602 0.651 0.627 0.491
    6.250.375 0.378 0.377 0.241
    3.120.279 0.285 0.282 0.146
    1.560.168 0.176 0.172 0.036
    00.131 0.141 0.136  
  • 数据处理:
  • 货期:
    3-5 working days

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相关问答

 常见问题解答
Q:

Please provide the following information regarding Rabbit Vascular Endothelial cell Growth Factor, VEGF ELISA Kit (CSB-E08524Rb)
a. Can this kit detect human VEGF?

A:
Thanks for your inquiry.
About Vascular Endothelial cell Growth Factor,there is VEGFA~D for human while there is only VEGF for rabbit.
CSB-E08524Rb This kit is designed according to: https://www.uniprot.org/uniprot/Q866G4.
It detects VEGF and it can not test human samples.
Pls let me know if you have any further questions. Thank you.