Canine Free Thyroxine,FT4 ELISA Kit

产品详情

产品描述：

CUSABIO犬游离甲状腺素（FT4）酶联免疫检测试剂盒（货号：CSB-E13196c），采用竞争法原理定量检测血清、血浆及组织匀浆样本中的FT4浓度。游离甲状腺素是评估犬类甲状腺功能的关键指标，参与调节代谢、生长发育等重要生理过程，其水平异常与甲状腺功能亢进或减退等疾病密切相关。本试剂盒检测范围为7.5-120 ng/mL，可覆盖犬科动物体内FT4的生理及病理浓度区间，实验操作基于竞争结合原理：样本中的FT4与酶标记抗原竞争结合包被抗体，通过显色反应强度与标准曲线比对实现精准定量。该试剂盒适用于科研领域中对犬类甲状腺功能的机制研究、疾病模型评估、营养代谢实验或药理学研究，能够为动物医学、比较内分泌学等基础研究提供可靠数据支持。试剂盒包含预包被板、标准品、检测抗体及显色系统等完整组分，需在2-8°C避光保存，建议实验前平衡至室温使用。
缩写：

FT4
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.5 ng/mL-120 ng/mL
灵敏度：

7.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-97
1:2	Average %	90
	Range %	88-100
1:4	Average %	95
	Range %	90-105
1:8	Average %	91
	Range %	85-102

回收率：

The recovery of canine FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-105
EDTA plasma (n=4)	95	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.