Pig free thyroxine (FT4) ELISA kit

产品详情

产品描述：

CUSABIO猪游离甲状腺素（FT4）酶联免疫检测试剂盒（货号：CSB-EQF027512PI），基于竞争法原理实现定量检测，适用于分析血清、血浆、细胞培养上清液及组织匀浆样本中的FT4水平，检测灵敏度范围为4-64 pmol/L。FT4是甲状腺分泌的重要激素，在调节机体代谢、生长发育及能量平衡中起核心作用，其游离态浓度直接反映甲状腺功能的活性状态。CUSABIO试剂盒采用竞争性免疫分析技术，通过标准品与样本中FT4竞争结合特异性抗体的原理，结合酶标反应实现精准定量，可为猪的生理病理研究、内分泌调控机制探索及药物干预实验提供可靠数据支持。科研人员可将其应用于动物营养代谢研究、环境应激评估、甲状腺相关疾病模型构建等领域，尤其适用于需要动态监测甲状腺功能变化的实验设计，如激素分泌调控、代谢性疾病机制研究或毒理学评价等，为畜牧兽医研究及生物医学基础科研提供标准化检测工具。
缩写：

FT4
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

4 pmol/L-64 pmol/L
灵敏度：

1.08 pmol/L
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-99
1:2	Average %	95
	Range %	87-106
1:4	Average %	96
	Range %	90-110
1:8	Average %	91
	Range %	86-98

回收率：

The recovery of pig FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-103
EDTA plasma (n=4)	96	93-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究进展：

FT4指的是游离甲状腺素，是指在血液中不与蛋白质结合的甲状腺素。最新研究发现，FT4水平与代谢综合征、糖尿病、心血管疾病等疾病密切相关。一项针对14,107名美国成年人的研究表明，FT4水平与冠状动脉钙化风险呈正相关关系。此外，一些研究表明，孕期FT4水平异常与胎儿神经发育相关的基因表达水平有关，提示FT4水平对胎儿神经发育的影响。