Mouse High density lipoprotein cholesterol, HDL-C ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO小鼠高密度脂蛋白胆固醇（HDL-C）定量检测试剂盒（货号：CSB-E12874m），采用双抗体夹心法原理设计，适用于血清、血浆及组织匀浆样本中HDL-C的精准检测。HDL-C作为脂质代谢关键指标，在胆固醇逆向转运、抗炎及抗氧化过程中发挥重要作用，其水平变化常与脂质代谢异常、动脉粥样硬化等病理机制相关。试剂盒检测灵敏度为7.8 ng/mL，线性范围覆盖7.8-500 ng/mL，可满足不同浓度样本的定量分析需求。实验操作简便，兼容常规酶标仪检测，适用于心血管疾病模型、肥胖或糖尿病研究中脂代谢动态监测、药物干预效果评估等科研场景。通过检测血清、血浆样本可系统性评估循环系统中HDL-C水平，而组织匀浆检测则有助于探索特定器官（如肝脏、脂肪组织）的局部脂质代谢调控机制，为代谢性疾病基础研究提供可靠工具。本试剂盒严格验证抗体特异性与批次稳定性，确保数据准确性和可重复性，适用于体外实验研究。
缩写：

HDL-C
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 ng/mL-500 ng/mL
灵敏度：

1.95 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	88
	Range %	84-92
1:2000	Average %	91
	Range %	85-97
1:4000	Average %	99
	Range %	95-104
1:8000	Average %	92
	Range %	88-96

回收率：

The recovery of mouse HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	97	91-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.