Mouse low density lipoprotein cholesterol (LDL-C) ELISA kit

产品详情

产品描述：

CUSABIO小鼠低密度脂蛋白胆固醇（LDL-C）酶联免疫检测试剂盒（货号CSB-EQ027860MO），适用于科研领域对小鼠血清、血浆及组织匀浆样本中LDL-C水平的定量分析。LDL-C是血液中胆固醇运输的主要形式，其异常升高与动脉粥样硬化、心血管疾病等病理过程密切相关，是脂质代谢研究的重要靶点。试剂盒基于竞争法原理设计，通过样本中的LDL-C与标记抗原竞争结合固相抗体，结合显色反应实现定量检测，灵敏度达0.312 μg/mL，线性检测范围为0.312-20 μg/mL。该产品可广泛应用于小鼠模型中的脂质代谢机制研究、心血管疾病相关药物开发、饮食干预实验或基因工程动物模型的表型分析，为评估脂质代谢异常、动脉硬化病理进程及潜在治疗策略提供可靠的实验工具。实验操作简便，支持多种生物样本检测，满足科研工作者对小鼠LDL-C水平的高通量、高特异性检测需求。
缩写：

LDL-C
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 μg/mL-20 μg/mL
灵敏度：

0.078 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	82-92
1:200	Average %	94
	Range %	91-98
1:400	Average %	107
	Range %	103-114
1:800	Average %	89
	Range %	85-94

回收率：

The recovery of mouse LDL-Cspiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	91	87-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.