Rat Low Density Lipoprotein cholesterol (LDL-C) ELISA kit

Rat Low Density Lipoprotein cholesterol (LDL-C) ELISA kit

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中文名称：

大鼠低密度脂蛋白胆固醇(LDL-C)酶联免疫试剂盒
货号：

CSB-E16561r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠低密度脂蛋白胆固醇（LDL-C）酶联免疫检测试剂盒（货号：CSB-E16561r），采用竞争法原理实现定量分析，适用于血清、血浆及组织匀浆样本中LDL-C含量的精准测定。LDL-C作为脂代谢关键指标，在心血管疾病机制研究及药物干预效果评估中具有重要科研价值。该试剂盒检测范围为125-8000 ng/mL，灵敏度高，可覆盖大鼠样本中LDL-C的常见浓度梯度，包含预包被微孔板、系列标准品、检测抗体及显色底物等核心组分，操作便捷且重复性良好。实验采用标准曲线定量法，通过竞争结合反应实现目标物浓度的精确计算，适用于实验室开展动脉粥样硬化模型评估、脂质代谢通路研究、降脂药物筛选等科研项目，为心血管疾病基础研究及营养学实验提供可靠数据支持。
缩写：

LDL-C
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

125 ng/mL-8000 ng/mL
灵敏度：

62.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	94-102
1:200	Average %	94
	Range %	91-97
1:400	Average %	102
	Range %	98-106
1:800	Average %	88
	Range %	84-92

回收率：

The recovery of rat LDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-99
EDTA plasma (n=4)	103	98-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
8000	0.089	0.088	0.089
4000	0.182	0.178	0.180
2000	0.376	0.369	0.373
1000	0.630	0.642	0.636
500	1.130	1.146	1.138
250	1.528	1.516	1.522
125	1.914	1.810	1.862
0	2.267	2.169	2.218

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Synergistic effects of pro-biotics or post-biotics with n-3 highly unsaturated fatty acids on chronic mild stress-induced dyslipidemia in rats J Lee, Y Jin, Y Park,Research Square,2024
The monomer of resveratrol butyrate ester ameliorates obesity and gut microbiota in high-fat diet rats YW Chen,Journal of Functional Foods,2024
Body composition and metabolic consequences of antibiotics most frequently administered to newborns in intensive care units: an experimental study in healthy newborn rats E Segura,Frontiers in medicine,2024
Ethanolic Extract of Orange Leaves Ameliorates Dyslipidemia in Streptozotocin-induced Diabetic Rats. N Dodantenna,/,2024
Body Composition and Metabolic Consequences of Most Frequent Antibiotics Given to Newborns in Intensive Care Units, an Experimental Study in Newborn Rats C Wang,/,2024
Alleviation of Limosilactobacillus reuteri in polycystic ovary syndrome protects against circadian dysrhythmia-induced dyslipidemia via capric acid and GALR1 signaling S Li,NPJ biofilms and microbiomes,2023
Eicosapentaenoic acid and docosahexaenoic acid, but not α-linolenic acid, decreased LDL-cholesterol synergistically with estrogen via regulation of cholesterol synthesis and clearance in ovariectomized rats ChungPark, et al,Nutrition research,2019
Palm Wine and Orijin Bitters Severed Hyperlipidemia and Immunomodulatory Responses in Atherogenic-diet Fed Male Wistar Rats Bukoye, Oyewo Emmanuel.et al,American Journal of Food Science and Health,2017
Effect of n-3 PUFA supplementation at different EPA: DHA ratios on the spontaneously hypertensive obese rat model of the metabolic syndrome Molinar-Toribio E et al,Br J Nutr,2015

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.