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  4. Rat Testosterone,T ELISA Kit

Rat Testosterone,T ELISA Kit

文献引用 (140) 说明书下载
  • 中文名称：
    大鼠睾酮(T)酶联免疫试剂盒
  • 货号：
    CSB-E05100r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠睾酮(T)酶联免疫试剂盒（CSB-E05100r）为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的T含量。大鼠睾酮（T）是男性激素，对生殖功能和男性特征发育至关重要。其合成主要在睾丸间质细胞（Leydig细胞）中进行，受促性腺激素（LH）调控。睾酮水平异常与多种生殖和生理问题相关，如少弱精子症、性功能障碍等。研究睾酮的作用机制有助于开发治疗相关疾病的药物和方法。试剂盒检测范围为0.13 ng/mL-25.6 ng/mL，灵敏度为0.06 ng/mL。动物实验研究中的激素水平监测，如生殖生物学机制探索、药物或环境因素对内分泌系统的影响评估、以及疾病模型（如代谢综合征或性腺功能障碍）的睾酮动态变化分析等领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    N/A
  • 缩写：
    T
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.13 ng/mL-25.6 ng/mL
  • 灵敏度：
    0.06 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat testosteronen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-96  
    1:2 Average % 97  
    Range % 92-104  
    1:4 Average % 85  
    Range % 81-88  
    1:8 Average % 89  
    Range % 83-93  
  • 回收率：
    The recovery of rat testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-95  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    25.6 0.238 0.236 0.237    
    6.4 0.435 0.431 0.433    
    2 0.611 0.615 0.613    
    0.5 0.984 0.982 0.983    
    0.13 1.403 1.398 1.401    
    0 2.001 2.003 2.002    
  • 本试剂盒所含材料：
      • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit Testosterone antibody.
      • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • Anti-Testosterone Antibody 1 x 6 ml -- Act as the detection antibody.
      • HRP-conjugated Testosterone 1 x 6 ml -- Bind to the DHT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
      • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
      • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
      • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
      • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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