Canine Testosterone (T) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬睾酮（Testosterone, T）是犬类体内重要的雄性类固醇激素，在生殖系统发育、代谢调节及肌肉生长中发挥关键作用。CUSABIO试剂盒采用竞争性酶联免疫吸附法（ELISA）原理，专为定量检测犬血清、血浆及组织匀浆样本中的睾酮含量而设计，检测灵敏度达0.1 ng/mL，线性范围为0.1-20 ng/mL，可精准覆盖生理及病理状态下的激素水平变化。试剂盒包含预包被特异性抗体的96孔板、标准品、检测抗体及显色系统，操作流程经过优化，可在4小时内完成检测，适用于实验室批量样本分析。该产品能够有效支持犬类生殖生理研究、内分泌疾病模型建立、睾丸功能评估以及药物干预实验中的激素水平监测，为动物营养学、兽医学及比较医学领域提供可靠的数据支持。所有组分均经过严格质控，建议实验前通过梯度稀释处理高浓度样本以确保检测结果准确性。
别名：

N/A
缩写：

T
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.1 ng/mL-20 ng/mL
灵敏度：

0.05 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine testosterone in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-97
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-89
1:8	Average %	89
	Range %	83-95

回收率：

The recovery of canine testosterone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	87	82-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.