Human Free Testosterone,F-TESTO ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

人游离睾酮(F-TESTO)酶联免疫试剂盒（CSB-E05096h）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的F-TESTO含量。人游离睾酮（F-TESTO）是睾酮的一种游离形式，对男性健康和生理功能至关重要。其研究背景涉及睾酮的生理作用、代谢途径及其与疾病如前列腺癌的关系。研究机制主要关注F-TESTO在体内的生物活性、代谢过程及其对健康的影响。试剂盒检测范围为7.5 pg/mL-1500 pg/mL，适用于精准分析低浓度样本。适用于科研场景下探究内分泌失调、多囊卵巢综合征、代谢综合征等疾病的分子机制，或评估运动生理学、衰老相关激素水平变化等研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

F-TESTO
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

7.5 pg/mL-1500 pg/mL
灵敏度：

3.75 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	94
	Range %	91-98
1:4	Average %	97
	Range %	93-101
1:8	Average %	94
	Range %	89-98

回收率：

The recovery of human F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	105	101-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.