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中文名称：大鼠游离睾酮(F-TESTO)酶联免疫试剂盒
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货号：CSB-E05097r
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规格：96T/48T
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价格：￥3900/￥2500
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其他：
产品详情
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产品描述：CUSABIO基于竞争法原理定量检测大鼠血清、血浆中游离睾酮（F-TESTO）的ELISA试剂盒，其检测灵敏度达0.3 pg/mL，线性范围为0.3-60 pg/mL，适用于精准分析微量游离睾酮水平。游离睾酮作为生物活性睾酮的主要形式，在大鼠生殖发育、性腺功能及代谢调控研究中具有重要价值，尤其在构建内分泌疾病模型或评估雄性激素相关生理机制时，该指标可灵敏反映睾丸间质细胞功能状态。试剂盒采用预包被特异性抗体的96孔板，通过生物素标记抗原与样本中游离睾酮竞争结合位点，配合链霉亲和素-HRP显色系统实现定量检测，实验全程仅需3.5小时，样本消耗量低至20 μL。该产品可为生殖生物学研究、环境内分泌干扰物评估、睾丸功能障碍模型验证以及雄激素相关药物作用机制探索提供可靠数据支持，广泛应用于神经内分泌调控、性腺衰老、代谢综合征等动物实验的激素水平检测，为科研工作者提供标准化的睾酮活性状态分析工具。
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缩写：F-TESTO
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma
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检测范围：0.3 pg/mL-60 pg/mL
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灵敏度：0.15 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Epigenetics and Nuclear Signaling
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 96 Range % 90-101 1:2 Average % 95 Range % 89-99 1:4 Average % 94 Range % 90-98 1:8 Average % 95 Range % 90-100 -
回收率：
The recovery of rat F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 89-104 EDTA plasma (n=4) 94 88-99 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 60 0.202 0.205 0.204 15 0.359 0.342 0.351 4.8 0.610 0.623 0.617 1.2 1.007 1.012 1.010 0.3 1.464 1.457 1.461 0 2.439 2.454 2.447 -
数据处理：
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货期：3-5 working days
引用文献
- Anacyclus pyrethrum enhances fertility in cadmium-intoxicated male rats by improving sperm functions AA Mahmoud, MAM Shaaban, WT Basal,BMC Complementary Medicine and Therapies,2024
- Hypoxia impairs male reproductive functions via inducing rat Leydig cell ferroptosis under simulated environment at altitude of 5000 m J Chai, S Liu, X Tian, J Wang, J Wen, C Xu,Life Sciences,2024
- Promising drug candidates for the treatment of polycystic ovary syndrome (PCOS) as alternatives to the classical medication metformin HS Anbar,European journal of pharmacology,2023
- Thiacloprid impairs reproductive functions of male Wistar rats AAN Mahmoud,NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY,2024
- Eugenol alleviates acrylamide-induced rat testicular toxicity by modulating AMPK/p-AKT/mTOR signaling pathway and blood–testis barrier remodeling M Moccio,Scientific reports,2024
- Protective effects of Vitamin E against Zinc Oxide nanoparticles-induced histotoxicity of liver and testicular tissue, genotoxicity and biomarker stress in male albino rats M Li,/,2024
- Benzophenone-2 exerts reproductive toxicity in male rats. Ż Broniowska,Reproductive toxicology,2023
- Rifaximin Protects against Malathion-Induced Rat Testicular Toxicity: A Possible Clue on Modulating Gut Microbiome and Inhibition of Oxidative Stress by Mitophagy NN Omar,Molecules,2022
- Down-regulation of insulin substrate receptor 1 and 2 in the liver may be a mechanism for insulin resistance during testosterone deprivation M Ghowsi,Journal of Basic and Clinical Pathophysiology,2021
- Attenuation of sleep deprivation dependent deterioration in male fertility parameters by vitamin C NI Rizk,Reproductive Biology and Endocrinology,2020
- Effect of Metformin and Flutamide on Insulin, Lipogenic and Androgen-Estrogen Signaling and Cardiometabolic Risk in a PCOS-prone Rodent Model Maria Kupreeva.et al,American Journal of Physiology-Endocrinology and Metabolism,2018
- The effects of milled Tribulus terrestris, Avena sativa, and white ginseng powder on total cholesterol, free testosterone levels and testicular tissue in rats fed a high-cholesterol diet Mehmet .et al,/,2018
- Restoration of Spermatogenesis Using a New Combined Herbal Formula of Epimedium koreanum Nakai and Angelica gigas Nakai in an Luteinizing Hyun Jun Park.et al,World J Mens Health,2017
- Prior caloric restriction increases survival of pre-pubertal obese-and PCOS-prone rats exposed to a challenge of time-limited feeding and physical activity. Diane A et al,J Appl Physiol,2013
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