Human Free Thyroxine,FT4 ELISA Kit

产品详情

产品描述：

本试剂盒为人游离甲状腺素（FT4）定量检测专用，采用竞争法酶联免疫吸附测定原理，适用于血清或血浆样本分析。游离甲状腺素是甲状腺激素的主要活性形式，参与调节机体代谢、生长发育等重要生理过程，其浓度变化可反映甲状腺功能状态。试剂盒检测范围为4-64 pmol/L，样本需提前进行适当离心处理并避免反复冻融，实验过程包含预包被抗体的微孔板、生物素化检测抗体及HRP标记物，通过标准曲线定量目标物浓度。该产品可应用于科研领域中对甲状腺功能调控机制的研究，例如甲状腺功能亢进/减退动物模型评估、环境因素对甲状腺激素代谢的影响分析，或药物干预实验中激素水平变化的动态监测，为内分泌系统相关基础研究提供可靠数据支持。
缩写：

FT4
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
检测范围：

4 pmol/L-64 pmol/L
灵敏度：

1.08 pmol/L
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-99
1:2	Average %	90
	Range %	87-100
1:4	Average %	95
	Range %	88-98
1:8	Average %	95
	Range %	86-98

回收率：

The recovery of human FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-98
EDTA plasma (n=4)	96	93-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.