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  4. Recombinant Arabidopsis thaliana Omega-6 fatty acid desaturase, endoplasmic reticulum (FAD2)

Recombinant Arabidopsis thaliana Omega-6 fatty acid desaturase, endoplasmic reticulum (FAD2)

  • 中文名称：
    拟南芥FAD2重组蛋白
  • 货号：
    CSB-CF342791DOA
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
    FAD2
  • Uniprot No.：
    P46313
  • 别名：
    FAD2; At3g12120; T21B14.6; T21B14_107; T23B7.6; Delta(12-fatty-acid desaturase; Fatty acid desaturase 2; Omega-6 fatty acid desaturase, endoplasmic reticulum
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-383
  • 氨基酸序列
    MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK ECIYVEPDREGDKKGVYWYNNKL
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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FAD2 Proteins

靶点详情

  • 功能：
    ER (microsomal) omega-6 fatty acid desaturase introduces the second double bond in the biosynthesis of 18:3 fatty acids, important constituents of plant membranes. Delta(12)-desaturase with regioselectivity determined by the double bond (delta(9) position) and carboxyl group of the substrate. Can use both 16:1 and 18:1 fatty acids as substrates. It is thought to use cytochrome b5 as an electron donor and to act on fatty acids esterified to phosphatidylcholine (PC) and, possibly, other phospholipids. Very low constitutive hydroxylation activity. Required for desaturation of fatty acids present in extraplastidial membranes, including mitochondria. Required for salt tolerance during seed germination and early seedling growth.
  • 基因功能参考文献：
    1. Data suggest that omega-6 desaturation is largely controlled by cis-acting sequence variants in the fatty acid desaturase 2 (FAD2) 5' untranslated region intron that determine the expression level of the gene. PMID: 28108698
    2. FAD2 and FATB may have influenced the adaptive evolution of seed oil composition to optimize seed oil melting point relative to germination temperatures PMID: 26865732
    3. Results suggest that FAD2 mediated high-level vacuolar and plasma membrane fatty acid desaturation is essential for the proper function of membrane attached Na+/H+ exchangers. PMID: 22279586
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Fatty acid desaturase type 1 family
  • 组织特异性：
    Expressed in shoots and roots. Expressed in leaves, stems, flowers and siliques.
  • 数据库链接：

    KEGG: ath:AT3G12120

    STRING: 3702.AT3G12120.1

    UniGene: At.23898

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